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Independent Laboratory Evaluation of the
3M™ Molecular Detection Assay 2 -
E. coli
O157:H7
for the Detection of
E. coli
O157:H7 in Raw Ground Beef
The 3M
™
Molecular Detection Assay 2 (MDA 2) –
E. coli
O157 (including H7) employs
real-time isothermal technology for the rapid and accurate detection of
E. coli
O157
(including H7) from select food matrices. This study included a method comparison
evaluation of the 3M MDA 2 –
E. coli
O157:H7 to the USDA/FSIS-MLG 5.09
Detection,
Isolation and Identification of Escherichia coli O157:H7 from Meat Products and
Carcass and Environmental Sponges
for the detection of
E. coli
O157:H7 in raw ground
beef (73% lean). The 3M MDA 2 –
E. coli
O157:H7 was evaluated for its ability to
accurately detect
E. coli
O157:H7 from raw ground beef after 10 hours of incubation.
In the method comparison study, the 3M
™
MDA 2 -
E. coli
O157:H7 demonstrated no
statistically significant differences between presumptive and confirmed results (dPOD
CP
)
or between candidate and reference method results (dPOD
C
) for the evaluation of the
raw ground beef matrix.
Study Report Authors
Alison DeShields, Benjamin Bastin, Patrick Bird, M. Joseph Benzinger, Jr., Erin
Crowley, James Agin, and David Goins
Q Laboratories, Inc., Cincinnati, OH, USA 45214
Submitting Company
3M
™
Food Safety Department
3M
™
Center
Building 260-6B-01
St. Paul, MN 55144
Independent Laboratory
Q Laboratories, Inc.
1400 Harrison Ave
Cincinnati, OH 45214
United States
Scope of Methods
(a) Target organism – E. coli
O157
(b) Matrix