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Materials and Methods
Test Kit Information
(a) MDA 2 –
E. coli
O157 Test Kits
(b) MDA 2 Software
(c) 3M BPW ISO Formulation
(d) Matrix Control Kits
Additional Supplies and Reagents
(a) 3M Molecular Detection System (instrument)
(b) 3M Molecular Detection Speed Loader Tray
(c) 3M Molecular Detection Heat Block Insert
(d) 3M Molecular Detection Cap/Decap Tool [Reagent]
(e) 3M Molecular Detection Cap/Decap Tool [Lysis]
(f) Buffered Peptone Water, ISO formulation (ISO BPW)
Apparatus
(a) Top Loading Balance – capable of weighing and measuring
25 – 2000 grams.
(b) Filter laboratory blender bags.
(c) Laboratory paddle blender - Seward 3500 or equivalent: for sample
homogenization.
(d) Incubators – capable of maintaining 41.5 ± 1 °C
(e) Dry bath incubators – capable of maintaining 100 ± 1 °C.
(f) Calibrated Thermometers – capable of measuring, 41.5 ± 1 °C, and
100 ± 1 °C.
(g) Serological Pipette Bulbs (Automatic Pipette) – For sampling and
delivering of 1 mL - 10 mL.