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(h) Serological pipettes – Aerosol resistant.
(i) Microcentrifuge – For 1.5 mL microcentrifuge tubes.
(j) Adjustable, variable volume single pipette capable of sampling and
delivering 10-1000 µL.
(k) Adjustable, variable volume multichannel pipette capable of sampling
and delivering 30 µL.
(l) Micropipette tips – Aerosol resistant.
(m) Sterile Gloves
(n) Felt-tip marker.
(o) Refrigerator – Capable of maintaining 2-8 °C.
(p) Freezer – Capable of maintaining -20 °C.
(q) Calibrated Timer
Reference Materials
Organisms used in the method comparison study were obtained from the following
sources:
(a)
American Type Culture Collection (ATCC)
—
Manassas, VA.
www.atcc.org.Safety Precautions
Enrichment
Shiga-toxin producing
Escherichia coli
is a Biosafety Level 2 organism.
Biological samples such as enrichments have the potential to transmit
infectious diseases. Follow all applicable local, state/provincial, and/or
national regulations on disposal of biological wastes. Wear appropriate
protective equipment which includes but is not limited to: protective eyewear,
face shield, clothing/lab coat, and gloves. All work should be conducted in
properly equipped facilities utilizing the appropriate safety equipment (for
example, physical containment devices). Individuals should be trained in
accordance with applicable regulatory and company/institution requirements