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28
Table 2016.2A (cont’d.)
: Comparative Results for the Detection of
E. coli
O157 in 325 g Raw Ground Beef (73% Lean) Test Portions by the
mericon
E.
1
coliO157Screen Plus using manual extraction vs. USDA/FSIS MLG 5.09 in a Collaborative Study (High Inoculum Level)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate presumptive
(CP)
Candidate confirmed (CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Ground
Beef
1
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
2
a
NA NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
3
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
4
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
5
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
6
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
7
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
8
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
9
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
10
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
MPN/
Test
Portion
5.13
3.45
7.62
11
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
12
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
13
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
Estimate
All
144 144
1.00
144 144
1.00
144
144
1.00
144 144
1.00
0.00
0.00
LCL
0.97
0.97
0.97
0.97
-0.03
-0.03
UCL
1.00
1.00
1.00
1.00
0.03
0.03
s
r
b
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.16
0.16
0.16
s
L
c
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.16
0.16
0.16
s
R
d
0.00
0.00
0.00
0.00
UCL
0.00
0.00
0.00
0.00
LCL
0.22
0.22
0.22
0.22
P
T
e
1.000
1.000
1.000
1.000
a
N/A – Laboratory did not participate or submit data for this matrix
3
b
Repeatability Standard Deviation,
c
Among-Laboratory Standard Deviation,
d
Reproducibility Standard Deviation,
e
P
T
Value = Homogeneity test of laboratory PODs
4
LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
5
6
7
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only