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25
Table 2016.1D
:Summary of Results for the Detection of
E. coli
O157:H7in 73% Lean Raw Ground Beef (325g)
1
Method
a
mericon
E. coli STEC O-Type
Extraction Method
Automated QIAsymphony
Inoculation Level
Un-inoculated
Low
High
Candidate Presumptive Positive/
Total # of Samples Analyzed
1/144
63/144
141/144
Candidate Presumptive POD (CP)
0.01(0.00, 0.04)
0.44(0.35, 0.52)
0.98(0.94, 0.99)
s
r
b
0.08(0.07, 0.16)
0.51(0.45, 0.52)
0.14(0.13, 0.16)
s
L
c
0.00(0.00, 0.03)
0.00(0.00, 0.16)
0.00 (0.00, 0.15)
s
R
d
0.08(0.08, 0.10)
0.51 (0.46, 0.52)
0.14(0.13, 0.16)
P Value
e
0.4368
0.8105
0.6042
Candidate Confirmed Positive/
Total # of Samples Analyzed
0/144
65/144
144/144
Candidate Confirmed POD (CC)
0.00 (0.00, 0.03)
0.45 (0.37, 0.54)
1.00 (0.97, 1.00)
s
r
0.00 (0.00, 0.16)
0.51 (0.45, 0.52)
0.00 (0.00, 0.16)
s
L
0.00 (0.00, 0.16)
0.00 (0.00, 0.15)
0.00 (0.00, 0.16)
s
R
0.00 (0.00, 0.22)
0.51 (0.46, 0.52)
0.00 (0.00, 0.22)
P Value
1.0000
0.8479
1.0000
Candidate Confirmed Positive/
Total # of Samples Analyzed
0/144
63/144
141/144
Candidate Presumptive Positive that
Confirmed POD (C)
0.00 (0.00, 0.03)
0.44 (0.35, 0.52)
0.98 (0.94, 0.99)
s
r
0.00 (0.00, 0.16)
0.51 (0.45, 0.52)
0.14 (0.13, 0.16)
s
L
0.00 (0.00, 0.16)
0.00 (0.00, 0.16)
0.00 (0.00, 0.15)
s
R
0.00 (0.00, 0.22)
0.51 (0.46, 0.52)
0.14 (0.13, 0.16)
P Value
1.0000
0.8105
0.6042
Positive Reference Samples/ Total #
of Samples Analyzed
0/144
65/144
144/144
Reference POD
0.00 (0.00, 0.03)
0.45 (0.37, 0.54)
1.00 (0.97, 1.00)
s
r
0.00 (0.00, 0.16)
0.51 (0.45, 0.52)
0.00 (0.00, 0.16)
s
L
0.00 (0.00, 0.16)
0.00 (0.00, 0.15)
0.00 (0.00, 0.16)
s
R
0.00 (0.00, 0.22)
0.51 (0.46, 0.52)
0.00 (0.00, 0.22)
P Value
1.0000
0.8479
1.0000
dLPOD (Candidate vs. Reference)
f
0.00 (-0.03, 0.03)
-0.01(-0.13, 0.10)
-0.02(-0.06, 0.01)
dLPOD (Candidate Presumptive vs.
Candidate Confirmed)
f
0.01(-0.02, 0.04)
-0.01(-0.13, 0.10)
-0.02(-0.06, 0.01)
a
Results include 95% Confidence Intervals,
r
Repeatability Standard Deviation,
c
Among-Laboratory Standard Deviation,
d
Reproducibility Standard Deviation
2
e
P Value = Homogeneity test of laboratory PODs;
f
A confidence interval for dLPOD that does not contain the value 0 indicates a statistical significant difference between
3
the two methods
4
5
6
7
8
9
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only