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36
Table 2016.2D (cont’d.)
: Comparative Results for the Detection of
E. coli
O157 in 325 g Raw Ground Beef (73% Lean) Test Portions by the
mericon
E.
1
coliSTEC O-Typeusing automated extraction vs. USDA/FSIS MLG 5.09 in a Collaborative Study (Low Inoculum Level)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate presumptive
(CP)
Candidate confirmed (CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Ground
Beef
1
12
5
0.42
12
5
0.42
12
5
0.42
12
5
0.42
0.00
0.00
2
a
NA NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
3
12
7
0.58
12
7
0.58
12
7
0.58
12
7
0.58
0.00
0.00
4
12
6
0.50
12
6
0.50
12
6
0.50
12
6
0.50
0.00
0.00
5
12
5
0.42
12
5
0.42
12
5
0.42
12
5
0.42
0.00
0.00
6
12
4
0.33
12
4
0.33
12
4
0.33
12
4
0.33
0.00
0.00
7
12
6
0.50
12
6
0.50
12
6
0.50
12
6
0.50
0.00
0.00
8
12
5
0.42
12
5
0.42
12
5
0.42
12
5
0.42
0.00
0.00
9
12
5
0.42
12
5
0.42
12
5
0.42
12
5
0.42
0.00
0.00
10
12
8
0.66
12
8
0.66
12
8
0.66
12
8
0.66
0.00
0.00
MPN/
Test
Portion
0.62
0.48
0.77
11
12
4
0.33
12
6
0.50
12
4
0.33
12
6
0.50
0.00
-0.17
12
12
3
0.25
12
3
0.25
12
3
0.25
12
3
0.25
0.00
0.00
13
12
5
0.42
12
5
0.42
12
5
0.42
12
5
0.42
0.00
0.00
Estimate
All
144
63
0.44
144
65
0.45
144
63
0.44
144
65
0.45
-0.01
-0.01
LCL
0.35
0.37
0.35
0.37
-0.13
-0.13
UCL
0.52
0.54
0.52
0.54
0.10
0.10
s
r
c
0.51
0.51
0.51
0.51
LCL
0.45
0.45
0.45
0.45
UCL
0.52
0.52
0.52
0.52
s
L
d
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.15
0.16
0.15
s
R
e
0.51
0.51
0.51
0.51
UCL
0.46
0.46
0.46
0.46
LCL
0.52
0.52
0.52
0.52
P
T
f
0.8105
0.8479
0.8105
0.8479
a
N/A – Laboratory did not participate or submit data for this matrix;
b
Results were not used in statistical analysis due to deviation from testing protocol.
3
c
Repeatability Standard Deviation,
d
Among-Laboratory Standard Deviation,
e
Reproducibility Standard Deviation,
f
P
T
Value = Homogeneity test of laboratory PODs
4
LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
5
6
7
8
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only