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37
Table 2016.2D (cont’d.)
: Comparative Results for the Detection of
E. coli
O157 in 325 g Raw Ground Beef (73% Lean) Test Portions by the
mericon
E.
1
coliSTEC O-Typeusing automated extraction vs. USDA/FSIS MLG 5.09 in a Collaborative Study (High Inoculum Level)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate presumptive
(CP)
Candidate confirmed (CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Ground
Beef
1
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
2
a
NA NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
3
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
4
12
11
0.92
12
12
1.00
12
11
0.92
12
12
1.00
-0.08
-0.08
5
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
6
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
7
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
8
12
11
0.92
12
12
1.00
12
11
0.92
12
12
1.00
-0.08
-0.08
9
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
10
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
MPN/
Test
Portion
5.13
3.45
7.62
11
12
11
0.92
12
12
1.00
12
11
0.92
12
12
1.00
0.00
-0.08
12
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
13
12
12
1.00
12
12
1.00
12
12
1.00
12
12
1.00
0.00
0.00
Estimate
All
144 141
0.98
144 144
1.00
144
141
0.98
144 144
1.00
-0.02
-0.02
LCL
0.94
0.97
0.94
0.97
-0.06
-0.06
UCL
0.99
1.00
0.99
1.00
0.01
0.01
s
r
c
0.14
0.00
0.14
0.00
LCL
0.13
0.00
0.13
0.00
UCL
0.16
0.16
0.16
0.16
s
L
d
0.00
0.00
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.05
0.16
0.05
0.16
s
R
e
0.14
0.00
0.14
0.00
UCL
0.13
0.00
0.13
0.00
LCL
0.16
0.22
0.16
0.22
P
T
f
0.6042
1.000
0.6042
1.000
a
N/A – Laboratory did not participate or submit data for this matrix;
b
Results were not used in statistical analysis due to deviation from testing protocol.
3
c
Repeatability Standard Deviation,
d
Among-Laboratory Standard Deviation,
e
Reproducibility Standard Deviation,
f
P
T
Value = Homogeneity test of laboratory PODs
4
LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
5
6
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only