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39

1

Table 1. Individual Collaborator Results for Raw Ground Beef (73% Lean) – Manual Extraction

High Level Test Portions

Low Level Test Portions

Un-inoculated Test Portions

1

2

3

4

5

6

7

8

9

10 11

12

1

2

3

4

5

6

7

8

9

10

11

12

1

2

3

4

5

6

7

8

9

10

11

12

mericon

E. coliO157 Screen Plus

Lab

a

1

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+

+

-

+

-

+

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

3

+

+

+ +

+

+ +

+

+ +

+

+

+

+

+

+

-

-

+

+

-

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

4

+

+

+ +

+

+ +

+

+ +

+

+

+ -

+

-

+

+

+

-

-

+

-

-

-

-

-

-

-

-

b

-

-

-

-

-

-

5

+

+

+ +

+

+ +

+

+ +

+

+

+

-

-

-

+

-

-

+

-

+

+

-

b

-

-

-

-

-

-

-

-

-

-

-

-

6

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+ +

-

+

-

-

-

-

-

+

-

-

b

-

-

-

-

-

-

-

-

-

-

7

+

+

+ +

+

+ +

+

+ +

+

+

-

-

-

b

-

+

+

-

-

+

+ +

+

-

-

-

-

-

-

-

-

-

-

-

-

8

+

+

+ +

+

+ +

+

+ +

+

+

-

+

-

+

-

+

-

+ +

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

9

+

+

+ +

+

+ +

+

+ +

+

+

+

-

-

+

-

-

-

-

-

+ +

+

-

-

-

-

-

-

-

-

-

-

-

-

10

+

+

+ +

+

+ +

+

+ +

+

+

-

+ +

-

+

+ + -

b

-

+ +

+

-

-

-

b

-

-

-

-

-

-

-

-

-

11

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+ + +

+

-

-

-

+ +

-

b

-

-

-

-

-

-

-

-

-

-

-

-

12

+

+

+ +

+

+ +

-

+ +

+

+

-

-

-

-

-

-

-

-

+

-

+

+

-

-

-

-

-

-

-

-

-

-

-

-

13

+

+

+ +

+

+ +

+

+ +

+

+

+ + + +

-

-

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

mericon

E. coliSTEC O-Type

Lab

a

1

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+

+

-

+

-

+

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

n/a

3

+

+

+ +

+

+ +

+

+ +

+

+

+

+

+

+

-

-

+

+

-

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

4

+

+

+ +

+

+ +

+

+ +

+

+

+ -

+

-

+

+

+

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

+

+

+ +

+

+ +

+

+ +

+

+

+

-

-

-

+

-

-

+

-

+

+

-

-

-

-

-

-

-

-

-

-

-

-

-

6

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+ +

-

+

-

-

-

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

7

+

+

+ +

+

+ +

+

+ +

+

+

-

-

-

-

+

+

-

-

+

+ +

+

-

-

-

-

-

-

-

-

-

-

-

-

8

+

+

+ +

+

+ +

+

+ +

+

+

-

+

-

+

-

+

-

+ +

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

9

+

+

+ +

+

+ +

+

+ +

+

+

+

-

-

+

-

-

-

-

-

+ +

+

-

-

-

-

-

-

-

-

-

-

-

-

10

+

+

+ +

+

+ +

+

+ +

+

+

-

+ +

-

+

+ +

-

-

+ +

+

-

-

-

-

-

-

-

-

-

-

-

-

11

+

+

+ +

+

+ +

+

+ +

+

+

-

-

+ + +

+

-

-

-

+ +

-

b

-

-

-

-

-

-

-

-

-

-

-

-

12

+

+

+ +

+

+ +

+

+ +

+

+

-

-

-

-

-

-

-

-

+

-

+

+

-

-

-

-

-

-

-

-

-

-

-

-

13

+

+

+ +

+

+ +

+

+ +

+

+

+ + + +

-

-

-

-

+

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

a

+=

E. coli

O157 was detected in samples; -=

E. coli

O157 was not detected in sample; n/a- lab did not participate or submit data for the matrix;

b

Sample was presumptive positive but confirmed negative indicating a false positive result;

OMAMAN-36 A : Collaborative Study Manuscript

For ERP Use Only

January 2017

AOAC Research Institute

Expert Review Panel Use Only