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Study Report Authors
Kiel Fisher, Benjamin Bastin, Jonathan Flannery, Allison Mastalerz, Patrick Bird,Erin
Crowley, M. Joseph Benzinger Jr., James Agin, David Goins
Q Laboratories, Inc., Cincinnati, OH 45214 USA
Method Authors
Marcia Armstrong, Kathrin Wolf, Sandra Luleyand Ralf Peist
Submitting Company
QIAGEN GmbH
QIAGEN Str. 1
40724 Hilden, Germany
Phone: +49-2103 292-0 (Germany)
Phone: 800-426-8157 (USA)
Fax: 800-718-2056 (USA)
Website:
www.qiagen.com(worldwide)
Independent Laboratory
Q Laboratories, Inc., Cincinnati, OH 45214 USA.
Reviewers
Yi Chen, US FDA Center for Food Safety and Nutrition
Elliot Ryser, Michigan State University
Yvonne Salfinger, Retired, Florida Department of Agriculture and Consumer Services
Scope of Method
(a)
Target organisms - E.coli O157 and E.coli non-O157 STECs(O26, O45,O103,
O111, O121, O145).
The
mericon
E. coli O157 Screen Plus Assay screens for
the presence of virulence factors
stx1/stx2, eae
andO157 while the
mericon
E.coliSTEC O-Type Assay detects the pathogenic STEC O-types
(O26, O45, O103, O111, O121, O145) or O157:H7.
(b)
Matrixes
1) Fresh spinach (25 g test portions)
2) Raw ground beef (70% lean) (325 g test portions)
3) Raw beef trim (325 g test portions)
(c)
Summary of validated performance claims
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only