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76

ReferenceMaterials

Organisms used in the inclusivity & exclusivity, robustness and method comparison

studies were obtained from the following sources:

(a)

American Type Culture Collection (ATCC; Manassas, VA)

(b)

National Culture Type Collection (NCTC; Salisbury, UK)

(c)

Michigan State University Culture Collection (The STEC Center, c/o Dr,Shannon

Manning, MSU; East Lansing, MI)

(d)

The Pennsylvania State University, E, coli Reference Center, Dr. Chitrita “Chobi”

DebRoy, University Park, PA

(e)

Q Laboratories Inc. Culture Collection (QL; Cincinnati, OH)

Organisms used in the lot-to-lot and product stability studies were obtained from:

(f)

ifp, Institut für Produktqualität GmbH, Dr.WolfgangWeber,Berlin, Germany,

Standard Solutions

(a)

1N Hydrochloric Acid (1N HCl)

Safety Precautions

mericon

E.coli O157 Screen Plus

and mericon

E.coli STEC O-Type

Kits

and

mericon

DNA Bacteria Kit

All chemicals should be considered potentially hazardous. When working with

chemicals, always wear a suitable lab coat and disposable gloves. Product usage

should follow good laboratory practices.

QIAsymphonymericonBacteria Kit

The buffers in the reagent cartridge contain guanidine salts, which can form highly

reactive compounds when combined with bleach. If liquid containing these buffers is

spilled, clean with a suitable laboratory detergent and water. If the spilled liquid contains

potentially infectious agents, clean the affected area first with laboratory detergent and

water, and then with 1% (v/v) sodium hypochlorite solution. Reagents in this kit are

highly flammable and are harmful by inhalation, skin contact and swallowing.

Real-Time PCR System

OMAMAN-36 E/ AOAC PTM Report

ERP Use Only

January 2017

AOAC Research Institute

Expert Review Panel Use Only