AOAC-RI ERP Book MICRO.pdf

Page

35)After the run is finished, select “Remove” in the assaysetup “Overview” screen.

Open the “Assays” drawer and unload the Rotor-Disc 72.

36)Place the Rotor-Disc sealing film over the Rotor-Disc72 and transfer to the

Rotor-Disc heat sealer. Sealing is complete in about 2 minutes. Remove the

excess film from the Rotor-Disc 72 and transfer to the Rotor-Disc 72

the

“Sample Rack Layout” screen of the assay setup user interface, the elution rack

in slot 2 is pictured.

Analysis

(a) Prepare Equipment

1) Turn on the Rotor-Gene Q instrument and attached computer. Load the

Rotor-Gene Q Series Software and templates for the

mericon

E. coli O157

Screen Plus and

mericon

E.coli STEC O-type assays. Start an empty run to

warm up the Rotor-Gene Q.

i. Run a Disc

ii. Load Samples

2) Manual mericonDNA Extraction Method

i. Load the QIAGEN strip tubes into the 72-Well Rotor strip tube ring.

Snap the locking ring in place over the strip tubes, and place into

the Rotor-Gene Q. When running strip tubes, balance the ring

using the empty strip tubes.

3) Automated QIAsymphonyDNA Extraction Method

i. Load the QIAGEN 72 Rotor-Disk into the 72-Rotor Disk ring. Snap

the locking ring in place over the Rotor Disk, and place into the

Rotor-Gene Q.

4) Start Instrument Run

i. Software Version 2.3.1 (Build 49)

Follow the Rotor-Gene software instructions. Verify that the correct

setting is selected for each preparation method: the Rotor-Disc 72

rotor is selected for the QIAsymphony (automated preparation

method), and the 72 count strip tubes for the manual preparation

method. Ensure that the locking ring is in place and select “Next”.

Verify that the reaction volume is 20 µL and select “Next”. Select

“Next” after verifying the temperature profile. Select “Start Run” to

start the Rotor-Gene Q instrument.

OMAMAN-36 E/ AOAC PTM Report

ERP Use Only

January 2017

AOAC Research Institute

Expert Review Panel Use Only