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Improper use of the Rotor-Gene Q may cause personal injuries or damage to the
instrument. The Rotor-Gene Q must only be operated by qualified personnel who
have been appropriately trained. Servicing of the Rotor-Gene Q must only be
performed by QIAGEN Field Service Specialists.
Enrichment
Shiga-toxin producing
E. coli
is a Biosafety Level 2 organism. Biological samples
such as enrichments have the potential to transmit infectious diseases. Follow all
applicable local, state/provincial, and/or national regulations on disposal of biological
wastes. Wear appropriate protective equipment which includes but is not limited to
protective eyewear, face shield, clothing/lab coat, and gloves. All work should be
conducted in properly equipped facilities utilizing the appropriate safety equipment
(for example, physical containment devices). Individuals should be trained in
accordance with applicable regulatory and company/institution requirements before
working with potentially infectious materials. All enrichment broths should be
sterilized following any culture-based confirmatory steps.
General Preparation
(a)
Use of aseptic techniques.
(b)
Use filter bags during enrichment to minimize particulates.
(c)
Clean the work stations with a disinfectant of choice
(e.g., sodium hypochlorite solution, phenol solution,Quaternary ammoniumsolution,
etc.)before and after use.
(d)
Separate work areas for the following: media preparation, sample preparation, DNA
lysis, and DNA amplification and detection.
(e)
Do not reuse kit disposables.
(f)
Change pipette tips between samples.
(g)
Wear personal protective equipment.
DNA Lysis
(a)
Pre-warm heat block to 100 ± 1 °C.
(b)
Change pipette tips between sample transfers.
(c)
Do not disrupt the pellet when discarding the supernatant.
(d)
Be sure to allow the samples to cool to room temperature (24 ± 2 °C) after the heat
lysis procedure.
DNA Amplification
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Revi w Panel Use Only