90-91 / 453
21
1
2
3
Table 1. Individual Collaborator Results for Raw Ground Beef (73% Lean)
High Level Test Portions
Low Level Test Portions
Un-inoculated Test Portions
1
2
3
4
5
6
7
8
9 10 11 12 1
2
3
4
5
6
7
8
9
10
11
12
1
2
3
4
5
6
7
8
9
10
11
12
3M MDA 2 -
E. coli O157 (including H7)
Lab
a
1
+ - - + - + + + + -
-
- + + - - - - - - -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
+ + + -
b
+ + + + + + + + - -
b
- + - + - - + -
- -
b
-
-
-
-
-
-
-
-
-
-
-
-
3
+ + + + + + + + + + + + - - + + + + + + +
c
+ + -
-
-
-
-
-
-
-
-
-
+
+
-
4
+ + + + + + + + + + + + - - - - - - - - - + - +
-
-
-
-
-
-
-
-
-
-
-
-
5
+
c
+ + + -
b
- + +
c
+ - + -
b
- - + - - - + - -
-
-
-
-
-
-
-
-
-
+
c
+
c
-
-
-
-
6
+ + + + + + + + + + + + + -
- - - - +
-
-
+
+
+
-
-
-
-
-
-
-
-
-
-
-
-
7
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
8
+ + + + + + + + + - + + - - - - - - + - - + + -
-
-
-
-
-
-
-
-
-
-
-
-
9
+ + + + + + - + + + + + - - - - - - - + + + -
-
-
-
-
-
-
-
-
-
-
-
-
-
10
+ - - - +
c
+
c
+
c
-
b
-
- + -
- + - - +
c
- - - -
- -
b
-
-
-
-
-
-
-
-
-
-
-
-
-
11
+ + +
c
- - - + + -
b
+ +
c
+ + + - - - - - - + -
b
-
- -
d
-
-
-
-
-
-
-
-
-
-
-
12
+ + + - + + + - + - + + - - + - - - - + + + -
-
-
-
-
-
-
-
-
-
-
-
-
-
13
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
14
+ -
- -
b
+ + + + + + + - + - + - - +
c
- - -
- +
c
-
-
-
-
-
-
-
-
-
-
-
-
-
15
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
USDA/FSIS – MLG 5.09
Lab
a
1
+ + + - + + + - + - + -
- - - + + - - - -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
+ - + + + + + + + + + + - + + - - - + - - + - +
-
-
-
-
-
-
-
-
-
-
-
-
3
+ + + + + + + + + + + + - - + + - + + + -
- + +
-
-
-
-
-
-
+
-
-
-
-
-
4
+ + + + + - - + - + + + + + - - - - + - + -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
+ - +
+
+
+
+
+
+
+
+
- -
-
-
-
-
-
+
+
+
-
-
+
- - - - - -
-
-
-
-
-
-
6
+ + + + + + + + + + - + + + - + - - - + + + +
-
-
-
-
-
-
-
-
-
-
-
-
-
7
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
8
+ + + + + + + + + + + + - - - - - - - - -
- + -
-
-
-
-
-
-
-
-
-
-
-
-
9
+ + - + + + + + + + + + + - - - - - - + -
- + +
-
-
-
-
-
-
-
-
-
-
-
-
10
- + - - - + - -
-
- + - + - - - - - - + -
-
- +
-
-
-
-
-
-
+
-
-
+
-
-
11
+ + - - + + + + + + + + + + + - - - - + -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
+ - - + - - +
+
-
-
-
-
- - - + - + - + + - + -
-
-
-
-
-
-
-
-
d
-
-
-
d
-
13
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
14
+ +
- + - + - + + + + + + + + - + + + - -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
a
+=
E. coli
O157 were detected in samples; -=
E. coli
O157 (including H7) were not detected in sample; n/a- lab did not participate or submit data for the matrix;
b
Sample was presumptive positive on 3M MDA 2 –
E. coli
O157 (including H7) but confirmed negative indicating a false positive result;
c
Sample was presumptive negative on 3M MDA 2 -
E. coli
O157 (including H7) but confirmed positive indicating a false negative result;
d
Original results were positive, revised after 16S rRNA
OMAMAN-35 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only