84-85 / 453
18
Table 2016.2A (cont’d.)
: Comparative Results for the Detection of
E. coli
O157:H7
in Raw Ground Beef (73% Lean) by the 3M
™
MDA 2 -
E. coli
O157
1
(including H7) Method vs. USDA/FSIS MLG Chapter. 5.09in a Collaborative Study (Low Inoculum Level)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate
presumptive (CP)
Candidate confirmed
(CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Ground
Beef
(73%
Lean)/
Low
1
12
2
0.167
12
2
0.167
12
2
0.167
12
2
0.167
0.00
0.00
2
12
5
0.417
12
3
0.250
12
3
0.250
12
5
0.417
-0.167
0.167
3
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
4
12
2
0.167
NA
2
0.167
NA
2
0.167
12
4
0.333
-0.167
0.00
5
12
2
0.167
NA
2
0.167
NA
2
0.167
NA
4
0.333
-0.167
0.00
6
12
5
0.417
12
5
0.417
12
5
0.417
12
7
0.583
-0.167
0.00
7
a
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
8
12
3
0.250
12
3
0.250
12
3
0.250
12
1
0.083
0.167
0.00
9
12
3
0.250
12
3
0.250
12
3
0.250
12
4
0.333
-0.083
0.00
10
a
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
MPN/
Test Portion
0.29
0.09-
0.55
11
12
4
0.333
12
3
0.250
12
3
0.250
12
4
0.333
-0.083
0.083
12
12
4
0.333
12
4
0.333
12
4
0.333
12
5
0.417
-0.083
0.00
13
a
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
14
12
2
0.167
12
4
0.333
12
2
0.167
12
6
0.500
-0.333
-0.167
15
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
Estimate
All
120 32
0.27
120 31
0.26
120 29
0.24
120 42
0.35
-0.11
0.01
LCL
0.19
0.18
0.16
0.26
-0.22
-0.10
UCL
0.35
0.34
0.32
0.44
0.01
0.12
s
r
b
0.45
0.45
0.44
0.48
LCL
0.40
0.40
0.39
0.42
UCL
0.52
0.52
0.51
0.52
s
L
c
0.00
0.00
0.00
0.05
LCL
0.00
0.00
0.00
0.00
UCL
0.16
0.13
0.13
0.23
s
R
d
0.45
0.45
0.44
0.48
UCL
0.40
0.40
0.39
0.43
LCL
0.52
0.51
0.50
0.52
P
T
e
0.760
0.920
0.908
0.342
a
N/A – Laboratory did not participate or submit data for this matrix,
b
Repeatability Standard Deviation,
c
Among-Laboratory Standard Deviation,
d
Reproducibility Standard Deviation,
3
e
P
T
Value = Homogeneity test of laboratory PODs,LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
4
OMAMAN-35 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only