82-83 / 453
17
Table 2016.2A
: Comparative Results for the Detection of
E. coli
O157:H7
in Raw Ground Beef (73% Lean) by the 3M
™
MDA 2 –
E. coli
O157 (including H7)
1
Method vs. USDA/FSIS MLG Chapter 5.09 in a Collaborative Study (Un-inoculated Control)
2
Statistic
Matrix/
Inoculation
Level
Laboratory
Candidate presumptive
(CP)
Candidate confirmed
(CC)
Candidate result (C)
Reference method (R)
C vs. R
N
X
POD
(CP)
N
X
POD
(CC)
N
X
POD
(C)
N
X
POD
(R)
dLPOD
(C,R)
dLPOD
(CP,CC)
Raw
Ground
Beef
(73%
Lean)/
Un-
inoculated
1
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
2
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
3
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
4
12
0
0.00
NA
0
0.00
NA
0
0.00
12
0
0.00
0.00
0.00
5
12
0
0.00
12
2
0.167
12
0
0.00
12
0
0.00
0.00
0.00
6
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
7
a
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
8
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
9
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
10
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
11
12
0
0.00
12
0
f
0.00
12
0
0.00
12
0
0.00
0.00
0.00
12
12
0
0.00
12
0
0.00
12
0
0.00
12
0
f
0.00
-0.00
0.00
13
a
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
14
12
0
0.00
12
0
0.00
12
0
0.00
12
0
0.00
0.00
0.00
15
NA NA
NA
NA NA
NA
NA NA
NA
NA NA
NA
NA
NA
Estimate
All
120
0
0.00
120
2
0.02
120
0
0.00
120
0
0.00
-0.000
0.017
LCL
0.00
0.00
0.00
0.00
-0.031
-0.059
UCL
0.03
0.06
0.03
0.03
0.031
0.017
s
r
b
0.00
0.12
0.00
0.00
LCL
0.00
0.11
0.00
0.00
UCL
0.17
0.17
0.17
0.17
s
L
c
0.00
0.04
0.00
0.00
LCL
0.00
0.00
0.00
0.00
UCL
0.17
0.09
0.17
0.17
s
R
d
0.00
0.13
0.00
0.00
UCL
0.00
0.12
0.00
0.00
LCL
0.24
0.16
0.24
0.24
P
T
e
1.00
0.031
1.00
1.00
a
N/A – Laboratory did not participate or submit data for this matrix
b
Repeatability Standard Deviation,
c
Among-Laboratory Standard Deviation,
d
Reproducibility Standard Deviation,
3
e
P
T
Value = Homogeneity test of laboratory PODs,
f
Original results identified this test sample(s) as
E. coli
O157:H7; reversed due to 16S rRNA results, LCL – Lower Confidence Limit; UCL – Upper Confidence Limit
4
5
OMAMAN-35 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only