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Table 2014.06B. Enrichment protocols using Demi-Fraser broth base without FAC
Sample matrix
Sample size
Primary enrichment, Demi-Fraser broth (no FAC)
Enrichment broth
volume, mL
Enrichment
temperature
(±1°C)
Enrichment
time, h
Food
Full-fat cottage cheese
25 g
225
37
24-28
Beef hot dogs
25 g
225
37
24-28
Deli turkey
25 g
225
37
24-28
Cold smoked salmon
25 g
225
37
24-28
Environmental surfaces
Stainless steel
1 Swab
10
37
26-30
Sealed concrete
1 Sponge
100
37
26-30
Stainless steel, sealed
concrete
1 Sponge
225
37
26-30
Candidates for 2016 Method of the Year
109