141 / 471
and the s
R
provides the variance in data between different laboratories. The P
T
value provides
1
information on the homogeneity test of laboratory PODs [11].
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
AOAC Official Method 2014.xxx
18
Listeria
speciesin Selected Foods and Environmental Surfaces
19
3M
™
Molecular Detection Assay (MDA)
Listeria
Method
20
First Action 2014
21
22
(Applicable to detection of
Listeria
species inselected foods including beef hot dogs (25 g), deli
23
turkey (25 g), cold smoked salmon (25 g), full fat cottage cheese (25 g), and two environmental
24
surfacessealed concrete (sponge in 100 mL and sponge in 225 mL enrichment volume) and
25
stainless steel (swab in 10 mL and sponge in 225 mL enrichment volume) and plastic (swab in
26
10 mL) enriched in Demi-Fraser broth base
27
See Table
2014.1
for a summary of results of the inter-laboratory study.
28
See Tables
2014.2
of the Supplementary Materialsfor detailed of results of the inter-laboratory
29
study
30
31
A.
Principle
32
33
3M
™
Molecular Detection Assay (MDA)
Listeria
method is intended for use with the 3M
™
34
Molecular Detection System for the rapid and specific detection of
Listeria
spp. in selected
35
foodsand environmental surfaces. The 3M MDA
Listeria
test uses isothermal amplification of
36
unique DNA target sequences with high specificity, efficiency, rapidity and bioluminescence to
37
detect the amplified sequences. Presumptive positive results are reported in real-time while
38
negative results are displayed after the assay is completed. Samples areenrichedin pre-warmed
39
3M
™
Demi Fraser (DF) broth base, which does not contain Ferric Ammonium Citrate (FAC).
40
41
B.
Apparatus and Reagents
42
Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA)
Listeria
kit
43
from 3M Food Safety (St. Paul, MN 55144-1000, USA).
44
(a)
3M Molecular Detection System
– Available from 3M Food Safety (St. Paul, MN
45
55144-1000, USA).
46
(b)
3M Molecular Detection Assay Listeria reagent tubes-
12 strips of 8 tubes. Available
47
from 3M Food Safety (St. Paul, MN 55144-1000, USA).
48
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
49
Candidates for 2016 Method of the Year
115