143 / 471
(c)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
1
Safety Precautions
2
Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools,
3
etc…) with a 1- 5% (v:v in water) household bleach solution or DNA removal solution.
4
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the
5
infirmed. It is recommended that these concerned groups avoid handling this organism.After
6
use, the enrichment medium and the 3M Molecular Detection Assay
Listeria
tubes can
7
potentially contain pathogenic materials. When testing is complete, follow current industry
8
standards for the disposal of contaminated waste. Consult the Material Safety Data Sheet for
9
additional information and local regulations for disposal.
10
Ethanol used in the method is flammable and caution should be used. Consult MSDS for
11
additional information.
12
13
D. Sample Enrichment
14
15
(a)
Pre-warmDF broth base without FAC,to 37 ±1°C.
16
(b)
Aseptically combine the enrichment medium and sample following the procedures in Table
17
Abelow. For all meat and highly particulate samples, the use of filter bags is recommended.
18
Homogenize thoroughly (Stomacher, blender) for 2 ± 0.5 minutes Incubate at 37 ±1°C.
19
20
21
22
23
Table A:
Enrichment protocols using
Demi-Fraser Broth Base without FAC
24
Sample Matrix
Sample
Size
Primary Enrichment
Demi-Fraser Broth (no FAC)
Enrichment
Broth Volume
(mL)
Enrichment
Temperatur
e (±1°C)
Enrichment
Time (hr)
Candidates for 2016 Method of the Year
117