(d)
Extra caps
– 12 strips of 8 caps
1
(e)
Negative control (NC)
- One vial (2 mL).
2
(f)
Reagent Control –
8 reagent tubes
3
(g)
Quick Start Guide
4
(h)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
5
(St. Paul, MN 55144-1000, USA).
6
(i)
3M
™
Molecular Detection Chill Block Tray and Chill block insert
- Available from
7
3M
™
Food Safety (St. Paul, MN 55144-1000, USA).
8
(j)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
9
(St. Paul, MN 55144-1000, USA).
10
(k)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
11
Food Safety (St. Paul, MN 55144-1000, USA).
12
(l)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
13
Food Safety (St. Paul, MN 55144-1000, USA).
14
(m)
Empty lysis tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
15
USA).
16
(n)
Empty reagent tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-
17
1000, USA).
18
(o)
Demi-Fraser Broth Base
– Available from 3M Food Safety (St. Paul, MN 55144-
19
1000, USA). (Formulation equivalent to ISO 11290-1:1996)
20
(p)
Disposable pipette
– capable of 20 µL
21
(q)
Multi
-channel (8-channel) pipette - capable of 20 µL
22
(r)
Sterile filter tip pipette tips
- capable of 20 µL
23
(s)
Filter Stomacher® bags –
Seward or equivalent
.
24
(t)
Stomacher
®– Seward or equivalent.
25
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
26
(v)
Dry double block heater unit or water bath –
capable of maintaining 100 ± 1
o
C
27
(w)
Incubators.
– Capable of maintaining 37 ± 1°C
28
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
29
Detection Chill Block Tray
30
(y)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection
31
Assay
32
(z)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
33
34
35
C. General Instructions
36
37
(a)
Store the 3M Molecular Detection Assay
Listeria
at 2-8°C. Do not freeze. Keep kit away
38
from light during storage. After opening the kit, check that the foil pouch is undamaged. If
39
the pouch is damaged, do not use. After opening, unused reagent tubes should always be
40
stored in the re-sealable pouch with the desiccant inside to maintain stability of the
41
lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 1 month. Do not use
42
3M Molecular Detection Assay
Listeria
past the expiration date.
43
(b)
The 3M Molecular Detection Instrument is intended for use with samples that have
44
undergone heat treatment during the assay lysis step, which is designed to destroy organisms
45
present in the sample. Samples that have not been properly heat treated during the assay lysis
46
step may be considered a potential biohazard and should NOT be inserted into the 3M
47
Molecular Detection Instrument.
48
Candidates for 2016 Method of the Year
116