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3M
™
Molecular Detection Assay (MDA)
Listeria monocytogenes
Method
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First Action 2014
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3
Applicable to detection of
Listeria monocytogenes
in (beef hot dogs (25 g & 125 g), deli turkey
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(25 g & 125 g), cold smoked salmon (25 g), full fat cottage cheese (25 g), chocolate milk (25 g),
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and two environmental surfaces, sealed concrete (sponge in 100 mL;sponge in 225 mL) and
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stainless steel (sponge in 225 mL).
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See Table
2014.1A-B
for a summary of results of the inter-laboratory study.
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See Tables
2014.2A-B
of the Supplementary Materialsfor detailed of results of the inter-
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laboratory study
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A.
Principle
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3M
™
Molecular Detection Assay (MDA)
Listeria monocytogenes
method is intended for use with
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the 3M
™
Molecular Detection System for the rapid and specific detection of
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Listeriamonocytogenes
in selected food and environmental surfaces. The 3M MDA
Listeria
17
monocytogenes
test uses isothermal amplification of unique DNA target sequences with high
18
specificity, efficiency, rapidity and bioluminescence to detect the amplified sequences.
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Presumptive positive results are reported in real-time while negative results are displayed after
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the assay is completed. Samples areenriched in pre-warmed Demi Fraser (DF) broth base, which
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does not contain Ferric Ammonium Citrate (FAC).
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B.
Apparatus and Reagents
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Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA)
Listeria
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monocytogenes
kit from 3M Food Safety (St. Paul, MN 55144-1000, USA).
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(a)
3M Molecular Detection System
– Available from 3M Food Safety (St. Paul, MN
27
55144-1000, USA).
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(b)
3M Molecular Detection Assay Listeria monocytogenes reagent tubes-
12 strips of 8
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tubes. Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
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(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
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(d)
Extra caps
– 12 strips of 8 caps
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(e)
Negative control (NC)
- One vial (2 mL).
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(f)
Reagent Control –
8 reagent tubes
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(g)
Quick Start Guide
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(h)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
36
(St. Paul, MN 55144-1000, USA).
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(i)
3M
™
Molecular Detection Chill Block Tray and Chill block insert
- Available from
38
3M
™
Food Safety (St. Paul, MN 55144-1000, USA).
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(j)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
40
(St. Paul, MN 55144-1000, USA).
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(k)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
42
Food Safety (St. Paul, MN 55144-1000, USA).
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(l)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
44
Food Safety (St. Paul, MN 55144-1000, USA).
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(m)
Empty lysis tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
46
USA).
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Candidates for 2016 Method of the Year
152