189 / 471
(n)
Empty reagent tube rack
- Available from 3M Food Safety (St. Paul, MN 55144-
1
1000, USA).
2
(o)
Demi-Fraser Broth Base (DF)
– Available from 3M Food Safety (St. Paul, MN
3
55144-1000, USA). (Formulation equivalent to ISO 11290-1)
4
(p)
Disposable pipette
– capable of 20 µL
5
(q)
Multi
-channel (8-channel) pipette - capable of 20 µL
6
(r)
Sterile filter tip pipette tips
- capable of 20 µL
7
(s)
Filter Stomacher® bags –
Seward or equivalent
.
8
(t)
Stomacher
®– Seward or equivalent.
9
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
10
(v)
Dry double block heater unit or water bath –
capable of maintaining 100 ± 1
o
C
11
(w)
Incubators.
– Capable of maintaining 37 ± 1°C
12
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
13
Detection Chill Block Tray
14
(y)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular Detection
15
Assay
16
(z)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
17
18
C. General Instructions
19
20
(a)
Store the 3M Molecular Detection Assay
Listeria monocytogenes
at 2-8°C. Do not freeze.
21
Keep kit away from light during storage. After opening the kit, check that the foil pouch is
22
undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes should
23
always be stored in the re-sealable pouch with the desiccant inside to maintain stability of the
24
lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 1 month. Do not use
25
3M Molecular Detection Assay
Listeria monocytogenes
past the expiration date.
26
(b)
The 3M Molecular Detection Instrument is intended for use with samples that have
27
undergone heat treatment during the assay lysis step, which is designed to destroy organisms
28
present in the sample. Samples that have not been properly heat treated during the assay lysis
29
step may be considered a potential biohazard and should NOT be inserted into the 3M
30
Molecular Detection Instrument.
31
(c)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
32
Safety Precautions
33
After use, the enrichment medium and the 3M Molecular Detection Assay
Listeria
34
monocytogenes
tubes can potentially contain pathogenic materials.
Listeria monocytogenes
is
35
of particular concern for pregnant women, the aged and the infirmed. It is recommended that
36
these concerned groups avoid handling this organism.When testing is complete, follow
37
current industry standards for the disposal of contaminated waste. Consult the Material Safety
38
Data Sheet for additional information and local regulations for disposal.
39
Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc…)
40
with a 1- 5% (v:v in water) household bleach solution or DNA removal solution.
41
D. Sample Enrichment
42
43
Candidates for 2016 Method of the Year
153