192 / 471
4.3
Repeat step 4.2 until each individual sample has been added to a corresponding LS tube in the
1
strip
2
4.4
Use the 3M Molecular Detection Cap/Decap Tool-Lysis to re-cap the LS tube strip. Use the
3
rounded side of the tool to apply pressure in a back and forth motion ensuring that the cap is
4
tightly applied.
5
6
7
8
4.5
Repeat steps 4.1 to 4.4 as needed, for the number of samples to be tested
9
4.6
When all samples have been transferred, then transfer
20 µL of NC into a LS tube
. Use the
10
3M Molecular Detection Cap/Decap Tool-Lysis tool to re-cap the LS tube.
11
4.7
Cover the rack of LS tubes with the rack lid and firmly invert 3-5 times to mix.
Suspension has
12
to flow freely inside the tube.
13
5.
Verify that the temperature of the 3M Molecular Detection Heat Block Insert is at 100 ±1°C. Place
14
the rack of LS tubes in the 3M Molecular Detection Heat Block Insert and heat for 15 ±1 minutes
15
(b)
. Samples that have not been properly heat treated during the assay lysis step may be considered a
16
potential biohazard and should NOT be inserted into the 3M Molecular Detection Instrument.
17
6.
Remove the rack of LS tubes from the heating block and allow to cool in the 3M Molecular
18
Detection Chill Block Insert for 10 ±1 minutes
(c)
.
Remove the rack lid during incubation on the
19
3M Molecular Detection Chill Block Insert
.
20
7.
Remove the rack of LS tubes from the 3M Molecular Detection Chill Block Insert/ 3M Molecular
21
Detection Chill Block Tray system. Replace the lid on the rack of LS tubes and firmly invert 3-5
22
times to mix.
Suspension has to flow freely inside the tube.
23
8.
Firmly tap the lysis tubes rack on the laboratory bench 3-5 times.
24
9.
Place the rack on the laboratory bench. Let it sit undisturbed for at least 5 minutes to allow the resin
25
to settle.
Do not mix or disturb the resin at the bottom of the tube
.
26
27
28
29
(a)
Alternatives to equilibrate the LS tubes to room temperature are to incubate the LS tubes in a 37
30
±1°C incubator for 1 hour or at room temperature overnight (16-18 hours).
31
(b)
An alternative to using dry heat for the lysis step is to use a water bath at 100 ±1°C. Ensure that
32
sufficient water is used to cover up to the liquid level in the LS tubes. Place the rack of LS tubes
33
in the water bath at 100 ±1°C and heat for 15
±1
minutes.
34
(c)
The LS solution may freeze when processing less than 48 LS tubes. Freezing of the LS solution
35
will not affect your test. If freezing is observed, allow the LS tubes to thaw for 5 minutes before
36
mixing.
37
38
J. A
MPLIFICATION
39
1.
One Reagent tube is required for each sample and the NC.
40
Candidates for 2016 Method of the Year
156