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Determination of B-Vitamins in Infant Formula and Adult/Pediatric Formulas
ESI LC-MSMS
4
Table-4
QQQ Parameters:
Compund
Name
Precursor
Ion
Product ion
Dwell
Time
Fragmentor
Voltage
CE
Cell Acc.
Voltage
Thiamine
265.2
122.2, 81.3
40
92
10, 30
7
Riboflavin
377.1
243.2, 198.1,
172.1
40
100
20, 35, 40
7
Niacinamide
123.2
80.3, 53.3
40
85
20, 30
7
Nicotinic acid
124.2
80.1, 78.1, 53.1
40
50
20, 20, 32
7
Pantothenic
acid
220.1
202.1, 90.0,
71.9
40
100
10, 10, 15
7
Pyridoxine
170.2
152.1, 134.1,
77.3
40
55
10, 20, 35
7
Biotin
245.2
227.3, 105.2,
97.1
45
110
10, 30, 30
7
Folic acid
442.2
295, 176, 120
45
110
10, 40, 40
7
d) Calculations:
(A
s
- A
b
) * V
s
* DF
Result (mg/100g) =
W
s
* 10000
A
s
= Sample concentration in μg/L, from calibration curve
A
b
= Blank concentration in μg/L, from calibration curve
V
s
= Sample Makeup Volume
W
s
= Sample weight in grams
DF
S
= Sample dilution factor (Other than V
s and
W
s
)
G.
References:
a)
AOAC official methods of analysis (AOAC 2004.07 , AOAC 2012.16 )
b)
BS EN 14122:2014, Food Stuffs
–
Determination of Vitamin B1 by HPLC
c)
BS EN 14164:2014, Food Stuffs
–
Determination of Vitamin B6 by HPLC
d)
BS EN 15652:2009, Food Stuffs
–
Determination of Niacin by HPLC
e)
Agilent application note for “Determination of multi water soluble vitamins by LC
-
MS/MS”.
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE