156 / 696
ValidationParameter
TestGroupName
Date
TestName
DataPathFile
Matrix
Raw-DataFileRef.
Equiment
Analyst
SampleBlankNo.
RetentionTime (min)
m/z
Standard (PeakArea)
Blank (PeakArea)
Area%ofblank to
lowestcalibrant
(Criteria=<30%)
Lowestcalibrant
concentrationug/l
1
3.941
377.1 >243.2
4222.7
411.0
9.73
5.0
2
3.941
377.1 >243.2
4220.2
411.0
9.74
5.0
3
3.941
377.1 >243.2
4077.6
411.0
10.08
5.0
4
3.941
377.1 >243.2
4147.9
411.0
9.91
5.0
5
3.941
377.1 >243.2
4032.4
411.0
10.19
5.0
6
3.941
377.1 >243.2
4038.2
411.0
10.18
5.0
7
3.941
377.1 >243.2
4204.4
411.0
9.78
5.0
8
3.941
377.1 >243.2
4272.9
411.0
9.62
5.0
9
3.941
377.1 >243.2
4107.4
411.0
10.01
5.0
10
3.941
377.1 >243.2
4115.9
411.0
9.99
5.0
Mean
3.94
Mean
9.922
Concentration
Analyte Retention
Time (Minutes)
377.1>172.1
Ion Ratio
% Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
3.941
4348.308564
2375
0.546
54.6
Pass
Confirmed
Calibration standard 2
3.941
7748.884013
4810
0.621
62.1
Pass
Confirmed
Calibration standard 3
3.941
40826.16555
23723
0.581
58.1
Pass
Confirmed
Calibration standard 4
3.924
82574.58657
48278
0.585
58.5
Pass
Confirmed
Calibration standard 5
3.941
173711.2294
102048
0.587
58.7
Pass
Confirmed
Calibration standard 6
3.941
429749.2638
253557
0.590
59.0
Pass
Confirmed
Mean
3.938
SRM 1849a-1
3.941
88826.34
51682.00
0.58
58
0.003
Pass
Confirmed
SRM 1849a-2
3.924
86761.32
51306.00
0.59
59
-0.015
Pass
Confirmed
SRM 1849a-3
3.923
87018.09
50127.00
0.58
58
-0.015
Pass
Confirmed
SRM 1849a-4
3.941
87652.85
51262.00
0.58
58
0.003
Pass
Confirmed
SRM 1849a-5
3.941
86242.42
51809.00
0.60
60
0.003
Pass
Confirmed
SRM 1849a-6
3.941
87028.15
50820.00
0.58
58
0.003
Pass
Confirmed
Average ion ratio
0.59
Relative intensity (%of
basepeak)
%Toleranceplusorminus
Average intensity
59
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.41
>10-20
30
Upper limit
0.76
<10
30
Page 6
AOAC-SPIFAN-2012-VITAMINS
MethodBlank
TUV/BLR/F-360
LC-MS/MS
ManjunathK/PrasadBKVR
Specificity
B-ComplexVitamins
01.01.2015
Riboflavin
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE