162 / 696
Validation Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Equiment
Analyst
Sample Blank No.
Retention Time (min)
m/z
Standard (Peak Area)
Blank (Peak Area)
Area % of blank to
lowest calibrant
(Criteria = <30%)
Lowest calibrant
concentration ug/l
1
3.333
442.2>295
1323.4
187.0
14.13
2.0
2
3.333
442.2>295
1359.8
187.0
13.75
2.0
3
3.333
442.2>295
1363.8
187.0
13.71
2.0
4
3.333
442.2>295
1268.4
187.0
14.74
2.0
5
3.333
442.2>295
1310.4
187.0
14.27
2.0
6
3.333
442.2>295
1276.8
187.0
14.65
2.0
7
3.333
442.2>295
1325.8
187.0
14.10
2.0
8
3.333
442.2>295
1342.0
187.0
13.93
2.0
9
3.333
442.2>295
1518.9
187.0
12.31
2.0
10
3.333
442.2>295
1359.0
187.0
13.76
2.0
Mean
3.33
Mean
13.937
Concentration Analyte Retention
Time (Minutes)
442.2>176.0
Ion Ratio
% Relative
Intensity
RT Difference
(criteria = ± 0.2
minute)
Ion Ratios
Overall
(μg/kg)
Area (Q1)
Area (Q2)
Pass - Fail
Pass - Fail
Calibration standard 1
3.333
2975.057627
907
0.305
30.5
Pass
Confirmed
Calibration standard 2
3.333
5939.641659
1883
0.317
31.7
Pass
Confirmed
Calibration standard 3
3.333
30455.02702
9337
0.307
30.7
Pass
Confirmed
Calibration standard 4
3.333
63141.05518
19441
0.308
30.8
Pass
Confirmed
Calibration standard 5
3.333
135368.6573
41994
0.310
31.0
Pass
Confirmed
Calibration standard 6
3.333
365563.4524
112807
0.309
30.9
Pass
Confirmed
Mean
3.333
SRM 1849a-1
3.333
7844.17
2564.00
0.327
32.7
0.000
Pass
Confirmed
SRM 1849a-2
3.333
8277.19
2512.00
0.303
30.3
0.000
Pass
Confirmed
SRM 1849a-3
3.333
7934.49
2570.00
0.324
32.4
0.000
Pass
Confirmed
SRM 1849a-4
3.333
7983.66
2385.00
0.299
29.9
0.000
Pass
Confirmed
SRM 1849a-5
3.333
8153.55
2435.00
0.299
29.9
0.000
Pass
Confirmed
SRM 1849a-6
3.333
7529.87
2559.00
0.340
34.0
0.000
Pass
Confirmed
Average ion ratio
0.31
Relative intensity (%
of base peak)
% Tolerance plus or
minus
Average intensity
31
>50
30
Tolerance (%)
30.00
>20-50
30
Lower limit
0.22
>10-20
30
Upper limit
0.40
< 10
30
Page 12
Method Blank
TUV/BLR/F-360
LC-MS/MS
Manjunath K/Prasad BKVR
AOAC- SPIFAN-2012-VITAMINS
Specificity
B-Complex Vitamins
01.01.2015
Folic acid
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE