AOAC SPIFAN ERP Meeting Book (3-16-16)

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Validation Parameter

Test Group Name

Date

Test Name

Data Path File

Matrix

Raw-Data File Ref.

Equiment

Analyst

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

Area % of blank to

lowest calibrant

(Criteria = <30%)

Lowest calibrant

concentration ug/l

1

2.097

170.2>152.1

46838.4

695.0

1.48

5.0

2

2.097

170.2>152.1

46494.7

695.0

1.49

5.0

3

2.097

170.2>152.1

47375.1

695.0

1.47

5.0

4

2.097

170.2>152.1

45064.5

695.0

1.54

5.0

5

2.097

170.2>152.1

47882.5

695.0

1.45

5.0

6

2.097

170.2>152.1

45994.3

695.0

1.51

5.0

7

2.097

170.2>152.1

45994.5

695.0

1.51

5.0

8

2.097

170.2>152.1

46518.0

695.0

1.49

5.0

9

2.097

170.2>152.1

47576.3

695.0

1.46

5.0

10

2.097

170.2>152.1

47071.2

695.0

1.48

5.0

Mean

2.10

Mean

1.489

Concentration Analyte Retention

Time (Minutes)

170.2>134.1

Ion Ratio

% Relative

Intensity

RT Difference

(criteria = ± 0.2

minute)

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail

Calibration standard 1

2.843

52068.7609

37624

0.723

72.3

Pass

Confirmed

Calibration standard 2

2.843

93709.7676

68929

0.736

73.6

Pass

Confirmed

Calibration standard 3

2.843

445905.4511

333530

0.748

74.8

Pass

Confirmed

Calibration standard 4

2.843

801082.5817

609512

0.761

76.1

Pass

Confirmed

Calibration standard 5

2.843

1420134.093

1093000

0.770

77.0

Pass

Confirmed

Calibration standard 6

2.843

2771730.763

2129000

0.768

76.8

Pass

Confirmed

Mean

2.843

SRM 1849a-1

2.843

552992.20

431534.00

0.78

78.0

0.000

Pass

Confirmed

SRM 1849a-2

2.843

567524.26

444719.00

0.784

78.4

0.000

Pass

Confirmed

SRM 1849a-3

2.843

556656.83

439467.00

0.789

78.9

0.000

Pass

Confirmed

SRM 1849a-4

2.843

559318.84

446583.00

0.798

79.8

0.000

Pass

Confirmed

SRM 1849a-5

2.843

569052.39

447307.00

0.786

78.6

0.000

Pass

Confirmed

SRM 1849a-6

2.843

554270.79

444354.00

0.802

80.2

0.000

Pass

Confirmed

Average ion ratio

0.76

Relative intensity (% of

base peak)

% Tolerance plus or

minus

Average intensity

76

>50

30

Tolerance (%)

30.00

>20-50

30

Lower limit

0.53

>10-20

30

Upper limit

0.99

< 10

30

Page 10

AOAC- SPIFAN-2012-VITAMINS

Method Blank

TUV/BLR/F-360

LC-MS/MS

Manjunath K/Prasad BKVR

Specificity

B-Complex Vitamins

01.01.2015

Pyridoxin

2015\January\Vitamins\01.01.2015

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE