AOAC SPIFAN ERP Meeting Book (3-16-16)

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Validation Parameter

Test Group Name

Date

Test Name

Data Path File

Matrix

Raw-Data File Ref.

Equiment

Analyst

Sample Blank No.

Retention Time (min)

m/z

Standard (Peak Area)

Blank (Peak Area)

Area % of blank to

lowest calibrant

(Criteria = <30%)

Lowest calibrant

concentration ug/l

1

1.867

124.2>80.1

3069.0

653.0

21.28

5.0

2

1.867

124.2>80.1

3032.7

653.0

21.53

5.0

3

1.867

124.2>80.1

2779.1

653.0

23.50

5.0

4

1.867

124.2>80.1

3012.4

653.0

21.68

5.0

5

1.867

124.2>80.1

3343.1

653.0

19.53

5.0

6

1.867

124.2>80.1

3230.8

653.0

20.21

5.0

7

1.867

124.2>80.1

3161.2

653.0

20.66

5.0

8

1.867

124.2>80.1

2919.9

653.0

22.36

5.0

9

1.867

124.2>80.1

2933.1

653.0

22.26

5.0

10

1.867

124.2>80.1

3066.1

653.0

21.30

5.0

Mean

1.87

Mean

21.431

Concentration Analyte Retention

Time (Minutes)

124.2 > 78.1

Ion Ratio

% Relative

Intensity

RT Difference

(criteria = ± 0.2

minute)

Ion Ratios

Overall

(μg/kg)

Area (Q1)

Area (Q2)

Pass - Fail

Pass - Fail

Calibration standard 1

1.867

3013.099027

2907

0.965

96.5

Pass

Confirmed

Calibration standard 2

1.867

5670.810404

5539

0.977

97.7

Pass

Confirmed

Calibration standard 3

1.867

26674.59717

24536

0.920

92.0

Pass

Confirmed

Calibration standard 4

1.867

50125.53391

45199

0.902

90.2

Pass

Confirmed

Calibration standard 5

1.867

92926.1074

85044

0.915

91.5

Pass

Confirmed

Calibration standard 6

1.867

186487.7079

162873

0.873

87.3

Pass

Confirmed

Mean

1.867

Eezee paste-1

1.867

21884.60

20694.00

0.95

95

0.000

Pass

Confirmed

Eezee paste-2

1.867

21997.95

20833.00

0.95

95

0.000

Pass

Confirmed

Eezee paste-3

1.867

22494.65

21173.00

0.94

94

0.000

Pass

Confirmed

Eezee paste-4

1.867

22371.28

20886.00

0.93

93

0.000

Pass

Confirmed

Eezee paste-5

1.867

22131.94

20807.00

0.94

94

0.000

Pass

Confirmed

Eezee paste-6

1.867

21606.36

20389.00

0.94

94

0.000

Pass

Confirmed

Average ion ratio

0.93

Relative intensity (%

of base peak)

% Tolerance plus or

minus

Average intensity

93

>50

30

Tolerance (%)

30.00

>20-50

30

Lower limit

0.65

>10-20

30

Upper limit

1.20

< 10

30

Page 8

AOAC- SPIFAN-2012-VITAMINS

Method Blank

TUV/BLR/F-360

LC-MS/MS

Manjunath K/Prasad BKVR

Specificity

B-Complex Vitamins

01.01.2015

Nicotinic acid

2015\January\Vitamins\01.01.2015

BVit-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE