174 / 696
Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
1.867
124.2>80.1
5.617
Mean (μg/l)
5.58
2
1.867
124.2>80.1
5.533
SD
0.3774
3
1.867
124.2>80.1
4.943
LOD (Mean +3 sigma)
6.72
4
1.867
124.2>80.1
5.486
LOQ (Mean + 10sigma)
9.36
5
1.867
124.2>80.1
6.255
RSD %
6.76
6
1.867
124.2>80.1
5.994
Standard Conc. (μg/l)
5
7
1.867
124.2>80.1
5.832
Accuracy %
111.7
8
1.867
124.2>80.1
5.271
9
1.867
124.2>80.1
5.301
Accuracy = 70 - 125 %
10
1.867
124.2>80.1
5.611
RSD% = <15%
Page 24
Criteria
Caluclation
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
Nicotinic acid
2015\January\Vitamins\01.01.2015
01.01.2015
Aqueous
TUV/BLR/F-360
TUV/BLR/F-99
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE