176 / 696
Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
2.097
170.2>152.1
5.655
Mean (μg/l)
5.64
2
2.097
170.2>152.1
5.611
SD
0.1082
3
2.097
170.2>152.1
5.723
LOD (Mean +3 sigma)
5.96
4
2.097
170.2>152.1
5.430
LOQ (Mean + 10sigma)
6.72
5
2.097
170.2>152.1
5.788
RSD %
1.92
6
2.097
170.2>152.1
5.548
Standard Conc. (μg/l)
5
7
2.097
170.2>152.1
5.548
Accuracy %
112.7
8
2.097
170.2>152.1
5.614
9
2.097
170.2>152.1
5.749
Accuracy = 70 - 125 %
10
2.097
170.2>152.1
5.685
RSD% = <15%
Page 26
Criteria
Caluclation
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
B-Complex vitamins
pyridoxin
2015\January\Vitamins\01.01.2015
01.01.2015
Aqueous
TUV/BLR/F-360
TUV/BLR/F-99
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
Manjunath K/Prasad BKVR
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE