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Validation
Parameter
Test Group Name
Date
Test Name
Data Path File
Matrix
Raw-Data File Ref.
Limit
CRM Data File Ref.
Equipment
Analyst
Test Method
Operator
Repeatability at LOQ 5 ug/l standard
Sample Blank No.
Mean Retention Time (min)
m/z
Signal (ug/l)
1
3.941
377.1 >243.2
5.465
Mean (μg/l)
5.35
2
3.941
377.1 >243.2
5.462
SD
0.1195
3
3.941
377.1 >243.2
5.257
LOD (Mean +3 sigma)
5.71
4
3.941
377.1 >243.2
5.358
LOQ (Mean + 10sigma)
6.55
5
3.941
377.1 >243.2
5.192
RSD %
2.23
6
3.941
377.1 >243.2
5.201
Standard Conc. (μg/l)
5
7
3.941
377.1 >243.2
5.439
Accuracy %
107.0
8
3.941
377.1 >243.2
5.537
9
3.941
377.1 >243.2
5.300
Accuracy = 70 - 125 %
10
3.941
377.1 >243.2
5.312
RSD% = <15%
Page 22
Criteria
Caluclation
Prasad BKVR/Kesav Kumar N
AOAC- SPIFAN-2012-VITAMINS
Limit of Quantification
TUV/BLR/F-360
TUV/BLR/F-99
Manjunath K/Prasad BKVR
B-Complex vitamins
Riboflavin
Aqueous
LC-MS/MS 6460A (BLR/PS-CRS/006.03)
01.01.2015
2015\January\Vitamins\01.01.2015
BVit-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE