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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
18
(% RSD range = 3.2-18.7%) as well as interday precision (% RSD = 15.6%) of the method over a
period of about 3 months was found to be satisfactory.
Table 10: 5-methyl-THF analysis in BCR 485 (dried vegetable powder) over a period of about 3
months
5-CH3-THF Results 09/11/12 09/14/12 09/18/12 09/21/12 10/10/12 10/12/12 10/19/12
mcg/g
Mean
2.291
2.453
2.179
2.530
2.900
2.881
2.881
%RSD
3.4%
0.9%
1.5%
3.2%
1.7%
-
2.1%
N =
3
3
3
3
3
1
3
5-CH3-THF Results 10/26/12 11/07/12 11/13/12 12/27/12 Grand
Grand
SD
mcg/g
Mean
2.949
2.613
2.465
2.708
2.615
0.249
%RSD
0.4%
2.2%
0.5%
3.2%
9.5
N =
3
3
3
6
35
35
Indicative Value*
2.14 + 0.42
*BCR Indicative Value, is not a certified value obtained by BCR analysis using a LC fluorescence
method and hog kidney conjugase based extraction and SPE clean-up.
Table 11: 5-formyl-THF analysis in BCR 485 (dried vegetable powder) over a period of about 3
months
5-CHO-THF
Results
09/11/12
09/14/12
09/18/12
10/19/12
10/26/12
11/02/12
mcg/g
Mean
0.063
0.070
0.073
0.065
0.059
0.071
%RSD
10.6%
51.1%
12.1%
2.9%
18.7%
12.2%
N =
3
3
3
3
3
3
5-CHO-THF
Results
11/07/12
11/13/12
12/27/12
Grand
Grand SD
mcg/g
Mean
0.075
0.052
0.054
0.063
0.010
%RSD
5.2%
13.0%
3.2%
15.6%
N =
3
3
6
25
25
There is no value provided for 5-formyl-THF for this sample by BCR
Total folate as folic acid results obtained for the BCR 485 (dried vegetable powder) by the method
over a period of about 3 months are presented in Table 12 The results obtained were quite
consistent over period of analysis. Values obtained for total folate as folic acid was about 96% of
the lowest range specified for this reference material. Intraday precision (% RSD range = 2.2-
3.3%) as well as interday precision (% RSD = 12.5%) of the method over a period of about 3
months was found to be satisfactory.
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE