AOAC SPIFAN ERP Meeting Book (3-16-16)

Memo

Monique Steegmans

16001n-MSS-MSS-SLV FOS01 AOAC997.08 Beneo-Orafti, 1 Febr 2016, page 12/18

3.3 Limit of quantification (LOQ).

The LOQ was checked by adding fructans to a placebo infant formula product at the level of

0.03g/100g reconstituted final product.

At this low level recovery is ranging from 90 to 98% which falls within the requirements of the

SMPR 2014.002. From these results we can conclude that the fructan content can be analyzed

with an acceptable repeatability.

4. Conclusion.

The method used for this SLV meets the requirements as described in the AOAC SMPR 2014.002

for fructans in Infant Formula and Adult/pediatric Nutritional Formula in which fructan is present in a

concentration ranging from 0.03 to 5.0 g/100g reconstituted final product.

Method performance requirements

SMPR 2014.002

Analytical range

0.03–5.0

Limit of quantitation

(LOQ)

≤0.03

Repeatability (RSDr)

≤6%

Recovery

90 to 110% of mean

spiked recovery over

the range of the assay

Reproducibility

(RSD

)

≤12%

The

precision,

observed during this SLV following FOS-01 meets the repeatability as proposed by

the workgroup, as the average RDSr is ≤ 6%. Like prescribed by the SLV the samples from the Kit,

fortified with fructan, were analyzed in double on 6 different days by different operators.

The

accuracy

was performed by spiking the Test Materials Kit samples by 3 different pure fructan

Sample

Units

J4G-00017 SPIFAN Control Milk Form EV4H2Q

Average

HP inulin (J4G-00012)

0,029 g/100 g reconstituted final product

added

Fructan

g/100g g

reconstituted

final product

0,028

0,027

0,028

0,027

recovery

98,0

90,5

98,0

93,2

94,9

Fos-01 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE