106 / 328
Primary and Secondary Evaluation of Method SJW-01
SJW-01:
Hypericumperforatum-Chemical Profling and Quantitative Results of St. John’s wort
Products by an Improved High-Performance Liquid Chromatography Method
Author(s):
M. Ganzera, J. Zhao, I.A. Khan, National Center for Natural Products Research, Research
Institute of Pharmaceutical Sciences, The University of Mississippi, University, Mississippi
38677, Department of Pharmacognosy, School of Pharmacy, The University of
Mississippi, University, Mississippi 38677
S
UMMARY OF
M
ETHOD
:
G
ENERAL
C
OMMENTS
:
The method is a fairly typical HPLC method used to quantify nine (9) main Hypericumperforatum
compounds in 35 minutes. The indicated compounds are hypericin, hyperforin, pseudohypericin, and six
(6) flavonoids: rutin, hyperoside, isoquercitrin, quercitrin, quercetin and 3, II8-Biapigenin. The identity
of each of the compounds was confirmed in an LC-MS experiment. The sample preparation for this
method involves methanol extraction (4 times), centrifugation and filtration.
P
ROS
/S
TRENGTHS
:
The method is simple and straightforward HPLC method and sample preparation. It was noted that the
manuscript has method validation data and the majority of well equipped analytical labs could run this
assay. The laboratory used five (5) commercially available standards and isolated three (3). At least two
of these standards, hypericin and hyperforin, are commercially available from Addipharma in Germany.
The I3, II8-biapigenin was quantified utilizing a relative response factor (according to literature and
relative to rutin).
C
ONS
/W
EAKNESSES
:
The manuscript does not contain detail regarding the types of samples tested (i.e., matrices not well
defined). The manuscript refers to commercial samples but does not give details of the type of samples.
EXPERT REVIEW PANEL VOTE
AND
RECOMMENDATION
MOTION:Not to make a decision on the method at this time pending review of the SLV data.
Roman, Brown (Unanimous) – Motion Passed
ERP PROFILE SUMMARIES
83