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Primary and Secondary Evaluation of Method SJW-05
SJW-05:
Simultaneous Determination of Protopseudohypericin, Pseudohypericin,
Protohypericin, and HypericinWithout Light Exposure
Author(s):
Steven F. Baugh, Industrial Laboratories, 4045 Youngfield St, Wheat Ridge, CO 80033
S
UMMARY OF
M
ETHOD
:
G
ENERAL
C
OMMENTS
:
This manuscript does not describe a method of analysis for determination of analytes in St. John’s wort
per se, but rather examines the feasibility of employing a mathematical conversion for accurate
determination of the naphthodianthrones without light exposure by employing a simplified protocol in
combination with using a conversion factor.
P
ROS
/S
TRENGTHS
:
This method could be a way to simplify sample preparation.
C
ONS
/W
EAKNESSES
:
The paper does not describe a method of analysis. Data supporting use of a mathematical conversion
with a simplified extraction protocol is presented. The focus is naphthodianthrones and does not apply
to any other stated compounds of interest in St. John's wort.The method does not meet the
requirements stated in the SMPR.
EXPERT REVIEW PANEL VOTE
AND
RECOMMENDATION
MOTION: Not to consider this method for First Action Official Method status.
Brown, Reif(Unanimous) Motion Passed
ERP PROFILE SUMMARIES
87