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Existing Methods (General)
▪
HPLC :
Regular System
▪
Column :
XTerra RP
8
, 5
P
, 4.6x 250mm
▪
UV Detection:
257 nm
▪
Mobile Phases
A: 25 mM NaH
2
PO
4
buffer
▪
B: ACN
▪
Features
Easy and Simple to do; Short;
Low Cost; Reliable
HPLC Method for Potency and Purity Test
Existing Methods (General)
10
2
4
6
8
12
min
250
200
150
100
50
0
0
mAU
SAMe
A Typical Chromatogram of SAMe Tablets