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2
As an example below are the calibration curves for cystine, taurine and isoleucine.
Table 1: Calibration curve of cystine
Conc.
[pmol/µL]
Area ratio
x [Nva]
Error
[%]
0.5
1.03
27.73
2
3.99
0.01
4
7.78
2.26
6
11.43
1.79
8
14.98
0.79
10
18.18
1.67
Table 2: Calibration curve of taurine
Conc.
[pmol/µL]
Area ratio
x [Nva]
Error
[%]
0.25
0.25
0.73
0.5
0.51
1.30
1
1.00
0.28
5
5.00
0.07
10
10.14
1.36
15
14.89
0.70
20
19.79
1.03
25
25.09
0.37
30
30.14
0.48
Table 3: Calibration curve of isoleucine
Conc.
[pmol/µL]
Area ratio
x [Nva]
Error
[%]
0.25
0.26
2.93
0.5
0.51
2.20
1
1.02
1.76
5
5.08
1.56
10
10.29
2.86
15
15.05
0.30
20
20.07
0.36
25
25.28
1.10
30
30.42
1.41
40
40.90
2.25
50
51.60
3.20
0
10
20
0
5
10
15
Area ratio x [Nva]
Concentration [pmol/µL]
Cys2
0
10
20
30
40
0
10
20
30
40
Area ratio x [Nva]
Concentration [pmol/µL]
Tau
0
20
40
60
0
20
40
60
Area ratio x [Nva]
Concentration [pmol/µL]
Ile
AMINO-01
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