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REAGENTS AND MATERIALS
4.1
Reagents
AccQ•Tag Ultra derivatization Kit (P/N 186003886) (Water Corp, Milford, Massachusetts).
AccQ
•
Tag Ultra Eluent A concentrate (P/N 186003838).
AccQ•Tag Eluent B (P/N 186003839) or HPLC-grade acetonitrile.
Formic acid (only if HPLC-grade acetonitrile is used instead of AccQ•Tag Eluent B).
Phenol (Sigma Aldrich P5566) or equivalent.
3,3’-dithio-dipropionic acid (Sigma Aldrich, 109010) or equivalent.
Sodium hydroxide pellets, reagent grade (Merck 1.06498.1000).
Milli-Q purified water (Millipore) or equivalent.
Hydrochloric acid fuming 37% GR for analysis.
Amino acid standard containing the following 17 amino acids at 2.5 µmol/mL each (except L-cystine
at 1.25 µmol/mL): L-alanine, L-arginine, L- aspartic acid, L-cystine, L-glutamic acid, L-glycine, L-
histidine, L-isoleucine, L-leucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-
tyrosine (P/N088122, Waters or equivalent).
L-cystine (Sigma Aldrich 30199) or equivalent.
Taurine (Sigma Aldrich PHR1109) or equivalent.
L-Norvaline (Sigma Aldrich N7627) or equivalent.
4.2
Materials
AccQ•Tag Ultra C18 Column 2.1 x 100 mm, 1.7 μm (P/N 186003837, Waters).
Adjustable micropipettors (1, 10, 20, 200, and 1000 μL) and tips.
10 mL glass tube with screw cap.
12 x 32 mm glass screw neck vial (Waters, 186000384c).
1.5 mL Eppendorf tubes.
AMINO-01
FOR ERP/WORKING GROUP USE ONLY
DO NOT DISTRIBUTE