AOAC SPIFAN Review Team (August 2016)

114 / 220

Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
4
Full SLV Report from Nestle Research Centre
Véronique Spichtig 1, Kimberley Iwanoff2, Sean Austin1.
1. Nestlé Research Centre, Vers‐Chez‐Les‐Blanc, Lausanne, Switzerland
2. Nestlé Quality Assurance Centre, York, UK.
TABLE OF CONTENTS
summary ................................................................................................................................................. 2
1 Introduction.......................................................................................................................................... 5
2 Methodology and Trials ......................................................................................................................... 6
2.1. Samples ...........................................................................................................................................................6
2.2. Method............................................................................................................................................................7
2.3. Validation Design...........................................................................................................................................13
3 Results .................................................................................................................................................16
3.1. System Calibration.........................................................................................................................................16
3.2. Analytical Precision .......................................................................................................................................17
3.3. Method Accuracy ..........................................................................................................................................18
3.4. Limits of Detection & Quantification.............................................................................................................20
4 Conclusions ..........................................................................................................................................21
Appendices .............................................................................................................................................23
1. Example Chromatograms ............................................................................................................................. 23
2. Raw Data (Precision) .................................................................................................................................... 26
3. Raw Data (Trueness) .................................................................................................................................... 29
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE