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Dual Lab Validation of a Method for the Determination of Fructans in Infant 

Formula & Adult Nutritionals /        

25 Jul 2016

2 M E T H O D O L O G Y A N D T R I A L S 

2.1.

Samples 

A SPIFAN single laboratory validation material test kit was used. The kit contains representative 

products covering the scope of the project as well as a standard reference material (SRM 1849a).  

The kit contains 5 products described as placebo products and 14 products described as fortified 

products.  Five of the fortified products were found to contain FOS. It was also found that one of the 

placebo products contained FOS. In addition to the SPIFAN matrices, two Nestlé reference samples 

were analysed. The Nestlé samples were analysed without prior reconstitution. 

Table 1: Samples Used In This Study

Product Description 

Lot No 

NRC 

Reference 

Placebo Products 

1. Child Formula Powder 

00847RF00 

KBI‐00020 

2. Infant Elemental Powder 

00796RF 

KBI‐00028 

3. Adult Nutritional RTF, High Protein 

00821RF00 

KBI‐00029 

4. Adult Nutritional RTF, High Fat 

00820RF00 

KBI‐00030 

5. Infant Formula RTF, Milk Based 

EV4H2Q 

KBI‐00031 

Fortified Products 

1. SRM 1849a 

CLC10‐b 

KBI‐00032 

2. Infant Formula Powder Partially Hydrolyzed 

Milk Based 

410057652Z 

KBI‐00033 

3. Infant Formula Powder Partially Hydrolyzed 

Soy Based 

410457651Z 

KBI‐00034 

4. Toddler Formula Powder Milk Based 

4052755861 

KBI‐00035 

5. Infant Formula Powder Milk Based 

4044755861 

KBI‐00044 

6. Adult Nutritional Powder Low Fat 

00859RF00 

KBI‐00045 

7. Child Formula Powder 

00866RF00 

KBI‐00046 

8. Infant Elemental Powder 

00795RF 

KBI‐00047 

9. Infant Formula Powder FOS/GOS Based 

50350017W1 

KBI‐00053 

10. Infant Formula Powder Milk Based 

K16NTAV 

KBI‐00048 

11. Infant Formula Powder Soy Based 

E10NWZC 

KBI‐00052 

12. Infant Formula RTF Milk Based 

EV4H2R 

KBI‐00049 

13. Adult Nutritional RTF High Protein 

00730RF00 

KBI‐00050 

14. Adult Nutritional RTF High Fat 

00729RF00 

KBI‐00051 

Nestlé Products 

1. Milk Powder With Prebiotics 

Ref. 40C 

Ref. 40C 

2. Infant Formula DDP 3/2016 

Ref. 64A 

Ref. 64A 

VALIDATION REPORT

FOR ERP USE ONLY

DO NOT DISTRIBUTE