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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
32
Content
1.
Introduction ………………………………………………………………………………………………………………. 33
2.
Principle analytical method………………………………………………………………………………………… 33
3.
Analytical method…………………………………………………………………………………………..………….. 34
4.
Validation samples and pure fructan ingredients……………………………………………………….. 34
5.
Validation design………………………………………………………………………………………………………… 35
a.
Introduction…………………………………………………………………………………………………… 35
b.
Calibration fit…………………………………………………………………………………………………. 35
c.
LOD and LOQ………………………………………………………………………………………………….. 36
d.
Precision ………………………………………………………………………………………………………… 36
e.
Accuracy …………………………………………………………………………………………………………. 37
f.
Specificity ……………………………………………………………………………………………………….. 37
6.
Results …………………………………………………………………………………………………………………………. 37
a.
Introduction ……………………………………………………………………………………………………. 37
b.
Calibration fit ………………………………………………………………………………………………….. 39
c.
LOD and LOQ …………………………………………………………………………………………………… 40
d.
Precision …………………………………………………………………………………………………………. 41
e.
Accuracy …………………………………………………………………………………………………………. 42
f.
Specificity ……………………………………………………………………………………………………….. 44
7.
Conclusions …………………………………………………………………………………………………………………. 46
8.
Literature ……………………………………………………………………………………………………………………. 46
9.
Apendices
a.
Detailed method description ………………………………………………………………………….. 47
b.
Experimental data calibration fit …………………………………………………………………….. 56
c.
Experimental data LOD and LOQ …………………………………………………………………….. 59
d.
Experimental data precision ……………………………………………………………………………. 60
e.
Experimental data accuracy …………………………………………………………………………….. 63
VALIDATION REPORT
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