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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
37
e.
Accuracy (Trueness)
The SPIFAN demands for the determination of the accuracy or trueness the determination of the
spiked recovery of an appropriate sampling of SPIFAN matrices. In this study 6 fructan containing
samples (after reconstition) in the SPIFAN sample kit are selected for the accuracy study. In section 6d,
results of the precision study, the overall means of the fructan content in these samples have been
established already and those values will be applied in this part of the accuracy study. According to the
AOAC SLV instructions, the samples will be spiked at about 50% and 150% level of the original fructan
content as determined in precision study. The spiked and unspiked samples will be analyzed in
duplicate on each of 3 different days.
Each of the 3 days of this accuracy study another pure fructan ingredient will be applied for spiking.
The purity of the respective fructan ingredients are established by subjecting these three pure fructan
ingredients to the AOAC 997.08 analysis.
And each day another HPAEC‐PAD instrument will be applied for the analyses.
f.
Specificity
There are no specific SPIFAN demands concerning the determination of specificity of the method. In
principle all kind of glucose and/or fructose containing carbohydrates (oligo‐ and polysaccharides)
which are susceptible for hydrolysis into their corresponding monosaccharides by (side) activities of
the used fructanase in last enzymatic hydrolysis step in the protocol, are potential interfering
constituents. And also disaccharides/oligosaccharides which chromatographically show coinciding
peaks with fructose or glucose will interfere. Thus potentially interfering carbohydrates in the new
developed fructan method are amongst others free disaccharides (e.g. sucrose, maltitol, palatinose)
and oligosaccharides, (resistant) malto‐oligosaccharides (e.g. polydextrose), (soluble) starch, and GOS.
Therefore the specificity of the method is demonstrated by showing that the above mentioned
constituents don’t interfere with the FOS quantitation.
6.
Results
a.
Introduction
The SPIFAN sample kit contains 19 samples. It is known that not all samples contain fructan. In order
to establish which samples contain fructan and which not, the whole series of 19 samples are analyzed
in duplicate, being as two independent analyses performed at two different days by two different
technicians. The samples have been analyzed as received, thus no reconstitution has been applied
preceding these single analyses.
The results are summarized in table 2.
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