149 / 220
Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
39
Table 3
Fructan content (% m/m) in the applied fructan standards (duplicate
averages) measured with the AOAC 997.08 and with the submitted
SPIFAN methods.
Fructan standard
ingredient
AOAC 997.08
method
Submitted SPIFAN method
duplicate
s
average
duplicates
average
Oraftie HP
91.7
93.0
92.7
94.6 90.5
92.6
Oraftie P95
85.8
85.4
85.6
84.7 80.8
82.8
Nutraflora P95
86.0
86.4
86.2
88.6 85.6
87.1
The duplicate averages as measured with the AOAC 997.08 method are used in the standard addition
experiments for calculating the exact amount of added pure fructan.
b.
Calibration fit
In this method quadratic calibration models are used for quantifying the glucose and the fructose
contents. Glucose is the minor constituent in fructans. Therefore the working range for glucose is at
lower levels than for fructose. In the standard daily routine just 5 calibration samples for glucose and
fructose are applied, ranging for glucose from 0.05– 20 mg/l and for fructose from 0.90 – 90 mg/l.
For testing the calibration models 12 calibration samples are used. Two times with the ISC5000_E
instrument and one time with the ICS3000_D instrument the calibration models were established for
this validation study. The results are summarized in table 4.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE