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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
45
In the column “expressed in dry product” with “no A0 Correction” the erroneously measured fructan
content is for all constituents, except Vivinal GOS and Litesse II polydextrose, at the LOQ level or
slightly higher. In this case a blank correction gives a significant improvement., especially for Vivinal
GOS and Litesse II polydextrose. However it is an unrealistic situation that in the tested pure
constituents low contents of fructan should be analyzed. Moreover those pure ingredients are
certainly not included in the scope of this method
Thus it can be concluded that the specificity of this new method is good.
Table 8
Results of the specificity experiments
500 mg test portion of the
compound investigated
fructan content in % m/m
expressed in liquid sample with
12.5% of the compound added
expressed in dry product
no A0
correction
with A0
correction
no A0
correction
with A0
correction
Fibersol resistant maltodextrin
0,004
0,002
0,035
0,014
Soluble starch
0,010
0,009
0,077
0,069
Palatinose
0,005
0,004
0,037
0,033
Maltitol
0,002
0,001
0,015
0,007
Sucrose (250mg)
0,006
0,005
0,048
0,039
Sucrose (500 mg)
0,006
0,005
0,049
0,041
Sucrose (1000 mg)
0,004
0,003
0,029
0,023
Vivinal GOS
0,023
0,002
0,182
0,017
Litesse II polydextrose
0,034
0,007
0,271
0,058
Figure 1
Chromatograms of Vivinal GOS (left) and Litesse II polydextrose (right).
In blue the chromatogram with no A0 correction, in pink the chromatogram with A0 correction and in
black the standards arabinose (alternative internal standard), galactose, glucose, fructose, sucrose and
chitobiose (internal standard)).
7.4 8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
16.0
17.0
18.0
19.0
20.0
21.0
22.0
23.0
24.0
-15.4
-10.0
-5.0
0.0
5.0
10.0
15.0
20.0
25.0
30.0
35.0
40.0
44.4
Arabinose
Galactose
Glucose
Fructose
Sucrose
chitobiose
min
nC
7.4 8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
16.0
17.0
18.0
19.0
20.0
21.0
22.0
23.0
24.0
-15.4
-10.0
-5.0
0.0
5.0
10.0
15.0
20.0
25.0
30.0
35.0
40.0
44.4
Arabinose
Galactose
Glucose
Fructose
Sucrose
chitobiose
min
nC
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE