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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
46
7.
Conclusions
The submitted new analytical method for the determination of the fructan content in infant formula
and aldult/pediatric nutritional formula meets the requirements as given in SMPR 2001.002.
The established LOQ is equal or less than the target value of 0.03%. With the exception of the tested
sample with a fructan content at LOQ level, the repeatability is much better than the target of 6% and
the reproducibility is much better than the target of 12%. The average accuracy is of the method is
94%. This is within the SMPR target range 90 – 110%. However the range in the 48 accuracy
experiments was 85 – 114%, just a little bit wider than the target range. The specificity of the method
is good.
Last but not least, in this new method no correction whatever has to be made for the type of fructans.
Reducing and/or non‐reducing fructan end‐groups don’t affect the analytical result. This is a great
advantage over the AOAC 999.03 method. And just one chromatographic run is needed instead of
three runs in case of the AOAC 997.08 method
8.
Literature
1. AOAC official method 997.08. Fructans in food products, ion exchange chromatographic method, first
action 1997, final action 1999.
2. AOAC official method 997.08. Measurement of fructan in food, enzymatic/spectrophotometric
method, first action 1999, final action 2005.
3. Dennis Cuany, Thierry Bénet, Sean Austin. Development and single‐laboratory validation of a method
for the determination of total fructans in infant formula. Journal AOAC Int. 2010
93(1)
202‐212
4. AOAC SMPR 2014.002, Standard method performance requirements for fructans in infant formula and
adult/pediatric nutritional formula, Approved by AOAC Stakeholder Panel on Infant Formula and Adult
Nutritionals (SPIFAN). Final Version Date: March 18, 2014.
5. Appendix L: AOAC Recommended Guidelines for Stakeholder Panel on Infant Formula and Adult
Nutritionals (SPIFAN) Single‐Laboratory Validation, approved by the AOAC Expert Review Panel on
Infant Formula and Adult Nutritionals in September 2011.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE