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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
52
Table 3
First dilution of the primary standard for preparing the calibration standards S1, S2
and S3
Dilution for preparing
intermediate
calibration standard
Volume primary
calibration standard
(ml)
Volume demineralized
water (ml)
(S1)
0.1
4.9
(S2)
0.5
4.5
(S3)
2.5
2.5
Compose the calibration standards by pipetting according to the scheme in table 4
Table 4 Pipetting scheme for preparation of the calibration standards
Calibration
standard
Volume
intermediate
calibration
standard
(ml)
Volume
primary
calibration
standard
(ml)
Volume
chitobiose
stock
solution
(ml)
Volume
demineralized
water (ml)
Volume
20%
acetonitrile
solution
(ml)
Total
volume
(ml)
S1
0.8
0
0.4
0.1
13.2
14.5
S2
0.8
0
0.4
0.1
13.2
14.5
S3
0.8
0
0.4
0.1
13.2
14.5
S4
0
0.8
0.4
0.1
13.2
14.5
S5
0
1.6
0.4
0.1
12.4
14.5
The concentrations of the sugars in the intermediate calibration standards (S1), (S2) and (S3) are
calculated with the formula:
The concentration of the secondary standards S1, S2 and S3 is calculated with the formula:
The concentrations of the secondary calibration standards S4 and S5 are calculated with the formula:
In which:
C
stock
= concentration sugar (mg/l)
C
interm
= concentration intermediate calibration standard (mg/l)
C
stand
= concentration secondary calibration standard (mg/l)
V
stock
= volume stock solution in intermediate solution (ml)
V
interm
= volume intermediate solution in secondary standard solution.
5.0 = volume intermediate solution (ml)
14.5 = total volume secondary calibration standard (ml)
5/)
(
int
stock
stock
erm
C V C
5.14/)
(
int
int
tan
erm
erm
d s
C V C
5.14/)
(
tan
stock
stock
d s
C V C
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE