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Dual Lab Validation of a Method for the Determination of Fructans in Infant
Formula & Adult Nutritionals /
25 Jul 2016
56
ANNEX B Experimental data lack of fit quadratic calibration curves
Results calibration test 24 March 2016
Fructose calibration ISC 5000_E instrument
Fructose
range: 0.887 – 177.4 mg/l
y = 0.409 x
2
+ 17,534 x + 0.070 R
2
= 0.9999
fructose fructose
fructose
lack of fit
respons
conc
calculated
absolute
%
t.o.v. IS
mg/l
mg/l
0,0426
0,887
1,026
‐0,139
‐15,6
0,0619
1,331
1,376
‐0,045
‐3,4
0,114
2,218
2,327
‐0,109 ‐4,9
0,235
4,436
4,549
‐0,113 ‐2,5
0,473
8,873
9,017
‐0,144 ‐1,6
0,816
15,53
15,59
‐0,062
‐0,4
1,15
22,18
22,19
‐0,010
0,0
2,16
44,36
43,22
1,14
2,6
3,15
66,55
65,45
1,10
1,7
4,20
88,73
90,62
‐1,89 ‐2,1
5,82
133,1
133,2
‐0,1
‐0,1
7,34
177,5
177,1
0,4
0,2
Glucose calibration ISC 5000_E instrument
Glucose
range 0.054 – 21.78 mg/l
y = 0.052 x
2
+ 6.539 x +0.006 R
2
= 0.9999
fructose fructose
fructose
lack of fit
respons
conc
calculated
absolute
%
t.o.v. IS
mg/l
mg/l
0,0426
0,887
1,026
‐0,139
‐15,6
0,0619
1,331
1,376
‐0,045
‐3,4
0,114
2,218
2,327
‐0,109
‐4,9
0,235
4,436
4,549
‐0,113
‐2,5
0,473
8,873
9,017
‐0,144
‐1,6
0,816
15,53
15,59
‐0,062
‐0,4
1,15
22,18
22,19
‐0,010
0,0
2,16
44,36
43,22
1,14
2,6
3,15
66,55
65,45
1,10
1,7
4,20
88,73
90,62
‐1,89
‐2,1
5,82
133,1
133,2
‐0,1
‐0,1
7,34
177,5
177,1
0,4
0,2
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE