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M
S
= the sample weight, in g
A
Lut
= the peak area of all-
trans
-lutein in the sample chromatogram (AU)
A
A
= the peak area of apocarotenal in the sample chromatogram (AU)
RF
Lut
= the response factor for all-
trans
-lutein
Lut
cis
= (M
A
/M
S
) x ([A
13cisLut
+ A
13’cisLut
+ A
9cisLut
+ A
9’cisLut
]/A
A
) x (100/RF
Lut
)
Where Lut
cis
= the
cis
isomers of lutein in the sample (μg/100 g)
M
A
= the mass of apocarotenal added to the test sample, in μg
M
S
= the sample weight, in g
A
13cisLut
= the peak area of 13-
cis
lutein in the sample chromatogram (AU)
A
13’cisLut
= the peak area of 13′-
cis
lutein in the sample chromatogram (AU)
A
9cisLut
= the peak area of 9-
cis
lutein in the sample chromatogram (AU)
A
9’cisLut
= the peak area of 9′-
cis
lutein in the sample chromatogram (AU)
A
A
= the peak area of apocarotenal in the sample chromatogram (AU)
RF
Lut
= the response factor for all-
trans
-lutein
Lut
Total
= Lut
trans
+ Lut
cis
(g)
Calculate the contents of all-
trans
-β-carotene,
cis
isomers of β-carotene, and total β-carotene in
the test samples:
BC
trans
= (M
A
/M
S
) x (A
BC
/A
A
) x (100/ RF
BC
)
Where BC
trans
= the all-
trans
-β-carotene in the sample (μg/100 g)
M
A
= the mass of apocarotenal added to the test sample, in μg
M
S
= the sample weight, in g
A
BC
= the peak area of all-
trans
-β-carotene in the sample chromatogram (AU)
A
A
= the peak area of apocarotenal in the sample chromatogram (AU)
RF
BC
= the response factor for all-
trans
-β-carotene
BC
cis
= RF
BC
x (M
A
/M
S
) x ([(A
15cisBC
x 1.4) + (A
13cisBC
x 1.2) + A
9cisBC
+ A
XcisBC
]/A
A
) x (100/ RF
BC
)
Where BC
cis
= the
cis
isomers of β-carotene in the sample (μg/100 g)
RF
BC
= the response factor for all-
trans
-β-carotene
M
A
= the mass of apocarotenal added to the test sample, in μg
M
S
= the sample weight, in g
A
BC
= the peak area of all-
trans
-β-carotene in the sample chromatogram (AU)
A
15cisBC
= the peak area of 15-
cis
-β-carotene in the sample chromatogram (AU)
A
13cisBC
= the peak area of 13-
cis
-β-carotene in the sample chromatogram (AU)
A
9cisBC
= the peak area of 9-
cis
-β-carotene in the sample chromatogram (AU)
METHOD
FOR ERP USE ONLY
DO NOT DISTRIBUTE