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Linearity
Linearity of the relative responses of analyte concentrations was measured using a 5-point
standard curve on three different days. Correlation coefficients, visual inspection, residuals,
and relative errors of back-calculated concentrations were used for evaluation. Linearity of
the internal standard was also demonstrated.
The ranges for lutein and β-carotene (4-240 μg/100 mL) correlate to approximately 0.8-45
μg/100 g as fed for samples prepared for the lowest sample concentrations. With dilutions
specified in the method, the range can be extended to approximately 2250 μg/100 as fed. This
extends beyond the range of 1-1300 μg/100 g specified in the SMPR.
Table 1. Residuals for the internal standard
Apocarotenal
Conc
(μg/100 mL)
Residual
203.5
4.119148067
101.8
-7.399430255
40.7
-3.177165248
20.4
1.435593088
4.07
2.222225757
2.04
2.79962859
Figure 1. Linearity plot for apocarotenal.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE