88 / 220
Figure 10. SPIFAN Toddler formula.
Figure 11. SPIFAN High protein adult nutritional RTF.
Precision
All samples were prepared in triplicate and the SPIFAN samples with substantial carotenoid
concentrations were prepared a at least 12 times (3 replicates x 4 days). Intermediate
precision (RSD
iR
) was calculated as the relative standard deviation of all replicates.
Repeatability (RSD
r
) for triplicate samples was calculated as the relative standard deviation,
and for larger samples sizes it was estimated by performing a one-way ANOVA on the
intermediate precision data and calculating the standard deviation from the within-day
variance. Although the SMPR does not specify criteria for intermediate precision, the
requirements for repeatability and reproducibility are outlined in Table 6. For all
measurements above 1 μg/100 g, the method meets the precision requirements of the SMPR.
Table 6. Precision requirements from SMPR 2014.014.
Sample conc (μg/100 g)
Repeatability
Reproducibility
1-100
8%
15%
>100-1300
5%
10%
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE