94 / 220
Accuracy
There is no certified reference material for carotenoids in infant formula and adult nutritionals.
Accuracy was assessed by spike and recovery of all-
trans
lutein and β-carotene is various
matrices. Spiked and unspiked samples were measured in triplicate. The SMPR calls for 90-
110% recovery from spiked samples, and all data meets this requirement.
Table 9. Spike and recovery data for SRM 1849a. Samples were spiked with standards at the
internal standard step of sample preparation.
Native level
(μg/100 g)
Spike
(μg/100 g)
Recovery
RSD
Spike
(μg/100 g)
Recovery
RSD
Lutein
ND
1.4
105.5%
0.8%
13.8
99.6%
0.2%
β-Carotene
0.2
1.3
107.5%
0.9%
13.6
103.2%
0.3%
ND Not detected
Table 10. Spike and recovery data for SRM 1849a. Samples were spiked with FloraGlo 5%
lutein at the powder reconstitution step of sample preparation.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Lutein
ND
34.8
92.3%
0.8%
68.3
94.0%
1.9%
135.3
97.6%
1.6%
ND Not detected
Table 11. Spike and recovery data for AND High Protein Placebo. Samples were spiked with
FloraGlo 5% lutein at the initial sample dilution step.
Native
level
(μg/100
g)
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Spike
(μg/100
g)
Recovery RSD
Lutein
3.3
62.7
96.7%
0.8%
148.8
100.1%
0.3%
297.6
101.0% 0.3%
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE